Difference between revisions of "Registry/Measurement kit/Notebook/2007-8-14"

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(New page: *PCR cleaned NotI digests of 56 and 55 (EX, SX) *re-circularizing ligation. *Sent some sequencing requests: **E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,...)
 
 
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*PCR cleaned NotI digests of 56 and 55 (EX, SX)
 
*PCR cleaned NotI digests of 56 and 55 (EX, SX)
 
*re-circularizing ligation.
 
*re-circularizing ligation.
 +
*Transforming  into Top10.
 +
 +
*Made overnights of colonies expresing GFP: 55-SX-B0031, 55-SX-B43, 55-EX-B34 (2 of each)
  
 
*Sent some sequencing requests:
 
*Sent some sequencing requests:
 
**E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,6
 
**E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,6

Latest revision as of 18:23, 14 August 2007

  • PCR cleaned NotI digests of 56 and 55 (EX, SX)
  • re-circularizing ligation.
  • Transforming into Top10.
  • Made overnights of colonies expresing GFP: 55-SX-B0031, 55-SX-B43, 55-EX-B34 (2 of each)
  • Sent some sequencing requests:
    • E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,6