Difference between revisions of "Registry/Measurement kit/Notebook/2007-8-1"

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*Started digest of promoters (E/S).
 
*Started digest of promoters (E/S).
 
*Started another culture
 
*Started another culture
 +
*To do: ligate R0040 into the digest from the PCR and transform.
  
 
==I2056==
 
==I2056==
 
*Purified digests (NotI, E/X, S/X)
 
*Purified digests (NotI, E/X, S/X)
 +
*Re-sealed and transformed.
 +
*To do: start choosing some RBSs to test
  
 
==I2055==
 
==I2055==
 
*Running gel of BioBrick PCR.
 
*Running gel of BioBrick PCR.
 
[[Image:8-1.jpg|thumb|left]]
 
[[Image:8-1.jpg|thumb|left]]
 +
*(ladder, I2055-EX, I2055-SX)
 
*Both look right size.
 
*Both look right size.
 
*Restriction digests: E/X, S/X, and NotI.
 
*Restriction digests: E/X, S/X, and NotI.
 +
 +
==T9002==
 +
*Re-did scarring PCR of F2620.

Revision as of 10:53, 1 August 2007

I2057

  • Started digest of promoters (E/S).
  • Started another culture
  • To do: ligate R0040 into the digest from the PCR and transform.

I2056

  • Purified digests (NotI, E/X, S/X)
  • Re-sealed and transformed.
  • To do: start choosing some RBSs to test

I2055

  • Running gel of BioBrick PCR.
8-1.jpg
  • (ladder, I2055-EX, I2055-SX)
  • Both look right size.
  • Restriction digests: E/X, S/X, and NotI.

T9002

  • Re-did scarring PCR of F2620.