Difference between revisions of "Registry/Measurement kit/Notebook/2007-7-30"

From OpenWetWare
Jump to: navigation, search
 
Line 8: Line 8:
 
*R0040 + a strong promoter + a weak promoter
 
*R0040 + a strong promoter + a weak promoter
 
*Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
 
*Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
 +
*Using Joey's transformation of the promoters.
  
 
==I2055==
 
==I2055==
 
*Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)
 
*Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)

Latest revision as of 11:46, 30 July 2007

To Do

  • See about making competent DB3.1 cells
  • Test the top10 stock with a + control (pUC18)
  • See what we can do with BB'd E0240 & I2057

Promoter Testers

  • Should pick some promoters to drop in.
  • R0040 + a strong promoter + a weak promoter
  • Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
  • Using Joey's transformation of the promoters.

I2055

  • Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)