Difference between revisions of "Reagents for SPR"

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aliquote into 200&mu;L portions directly into vials to be used on the instrument<br>  
 
aliquote into 200&mu;L portions directly into vials to be used on the instrument<br>  
 
freeze the aliquotes and store at -20 for no more then 2 months<br>
 
freeze the aliquotes and store at -20 for no more then 2 months<br>
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 9.781 mL water
+
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 10 mL water
 
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|N-hydroxysuccinimide ('''NHS''')<br>
 
|N-hydroxysuccinimide ('''NHS''')<br>

Latest revision as of 14:23, 19 May 2008

reagents purpose Mr handling instructions
1-ethyl-3-(3-dimethyl

aminopropyl) carbodiimide (EDC)
ethanolamine

amine coupling 191.70 EDC stock solution 0.4M

resuspend fresh ultrapure reagent in milli-Q water
aliquote into 200μL portions directly into vials to be used on the instrument
freeze the aliquotes and store at -20 for no more then 2 months
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 10 mL water

N-hydroxysuccinimide (NHS)
amine coupling 115.09 NHS stock solution 0.1M

prepare and store as desribed for EDC
Biacore supplied 115 mg portion should be dissolved in 10 mL water