Difference between revisions of "Reagents for SPR"

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|EDC stock solution 0.4M<br>
 
|EDC stock solution 0.4M<br>
 
resuspend fresh ultrapure reagent in milli-Q water<br>
 
resuspend fresh ultrapure reagent in milli-Q water<br>
aliquote into 150&mu;L portions directly into vials to be used on the instrument<br>  
+
aliquote into 200&mu;L portions directly into vials to be used on the instrument<br>  
 
freeze the aliquotes and store at -20 for no more then 2 months
 
freeze the aliquotes and store at -20 for no more then 2 months
 
|-
 
|-

Revision as of 13:02, 19 May 2008

reagents purpose Mr handling instructions
1-ethyl-3-(3-dimethyl

aminopropyl) carbodiimide (EDC)
ethanolamine

amine coupling 191.70 EDC stock solution 0.4M

resuspend fresh ultrapure reagent in milli-Q water
aliquote into 200μL portions directly into vials to be used on the instrument
freeze the aliquotes and store at -20 for no more then 2 months

N-hydroxysuccinimide (NHS)
amine coupling 115.09 NHS stock solution 0.1M

prepare and store as desribed for EDC