Rao:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{Rao}} | {{Rao}} | ||
{|cellspacing="5" cellpadding="10" style="background:#3674C2; width: 750px;" | |||
|-valign="top" | |||
|style="background:#ffffff"| | |||
==<center>General Techniques</center>== | |||
*[[Rao:ColPCR | Colony PCR Verification]] | *[[Rao:ColPCR | Colony PCR Verification]] | ||
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]] | *[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]] | ||
*[[Rao:Mega-Primer | Mega-Primer]] | *[[Rao:Mega-Primer | Mega-Primer]] | ||
*[[Rao:Restriction_Digest | Restriction Digest]] | *[[Rao:Restriction_Digest | Restriction Digest]] | ||
*[[Rao:Ligation | DNA Ligation]] | *[[Rao:Ligation | DNA Ligation]] | ||
*[[Rao:BetaGal | Beta-galactosidase Assay]] | *[[Rao:BetaGal | Beta-galactosidase Assay]] | ||
*[[Rao:Tecan | Tecan Safire2]] | *[[Rao:Tecan | Tecan Safire2]] | ||
==<center>''E. coli/S. typhimurium'' specific</center>== | |||
*[[Rao:Transformation | DNA Transformation]] | *[[Rao:Transformation | DNA Transformation]] | ||
*[[Rao:Transduction | Transduction]] | *[[Rao:Transduction | Transduction]] | ||
Line 26: | Line 20: | ||
*[[Rao:CRIM | CRIM Plasmid Integration]] | *[[Rao:CRIM | CRIM Plasmid Integration]] | ||
==<center>''B. subtilis specific''</center>== | |||
*[[Rao:BsTransformation | B. subtilis transformation]] | *[[Rao:BsTransformation | B. subtilis transformation]] | ||
< | ==<center>Media Preparation</center>== | ||
*[[Rao:LB | LB]] | *[[Rao:LB | LB]] | ||
*[[Rao:SOBC | SOB/SOC]] | *[[Rao:SOBC | SOB/SOC]] | ||
Line 41: | Line 31: | ||
*[[Rao:MotilityPlates | Motility Plates]] | *[[Rao:MotilityPlates | Motility Plates]] | ||
If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here] | If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here] | ||
Revision as of 18:59, 16 December 2010
Rao Lab
|
|