Difference between revisions of "Rao:Protocols"

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==<center>General Techniques</center>==
  
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<h4>PCR</h4>
 
 
*[[Rao:ColPCR | Colony PCR Verification]]
 
*[[Rao:ColPCR | Colony PCR Verification]]
 
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]]
 
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]]
 
*[[Rao:Mega-Primer | Mega-Primer]]
 
*[[Rao:Mega-Primer | Mega-Primer]]
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<h4>Need-to-know Techniques</h4>
 
'''''General'''''
 
 
*[[Rao:Restriction_Digest | Restriction Digest]]
 
*[[Rao:Restriction_Digest | Restriction Digest]]
 
*[[Rao:Ligation | DNA Ligation]]
 
*[[Rao:Ligation | DNA Ligation]]
 
*[[Rao:BetaGal | Beta-galactosidase Assay]]
 
*[[Rao:BetaGal | Beta-galactosidase Assay]]
 
*[[Rao:Tecan | Tecan Safire2]]
 
*[[Rao:Tecan | Tecan Safire2]]
'''''E. coli/S. typhimurium specific'''''
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==<center>''E. coli/S. typhimurium'' specific</center>==
 
*[[Rao:Transformation | DNA Transformation]]
 
*[[Rao:Transformation | DNA Transformation]]
 
*[[Rao:Transduction | Transduction]]
 
*[[Rao:Transduction | Transduction]]
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*[[Rao:CRIM | CRIM Plasmid Integration]]
 
*[[Rao:CRIM | CRIM Plasmid Integration]]
  
'''''B. subtilis specific'''''
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==<center>''B. subtilis specific''</center>==
 
*[[Rao:BsTransformation | B. subtilis transformation]]
 
*[[Rao:BsTransformation | B. subtilis transformation]]
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<h4>Media Preparation</h4>
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==<center>Media Preparation</center>==
 
*[[Rao:LB | LB]]
 
*[[Rao:LB | LB]]
 
*[[Rao:SOBC | SOB/SOC]]
 
*[[Rao:SOBC | SOB/SOC]]
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*[[Rao:MotilityPlates | Motility Plates]]
 
*[[Rao:MotilityPlates | Motility Plates]]
 
If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here]
 
If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here]
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Revision as of 18:59, 16 December 2010

Rao Lab

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General Techniques

E. coli/S. typhimurium specific

B. subtilis specific

Media Preparation

If your interested in more recipes go here