RNA extraction using trizol/tri: Difference between revisions
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RNA extraction with TRIZOL (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total [[RNA extraction]] from cells. It takes slightly longer than column-based methods like RNAeasy but it has higher capacity and can yield more RNA. | |||
== Principle == | == Principle == | ||
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* [http://www.animal.ufl.edu/hansen/protocols/RNA_extraction.htm RNA extraction with TRIZOL] (Uni Florida) | * [http://www.animal.ufl.edu/hansen/protocols/RNA_extraction.htm RNA extraction with TRIZOL] (Uni Florida) | ||
* [http://www.utm.utoronto.ca/~w3flyma/protocols/Trizol%20Troubleshooting.pdf Troubleshooting guide for TRIZOL extraction] (Uni Toronto) | * [http://www.utm.utoronto.ca/~w3flyma/protocols/Trizol%20Troubleshooting.pdf Troubleshooting guide for TRIZOL extraction] (Uni Toronto) | ||
Tips | |||
* [http://www.ambion.com/techlib/tn/94/949.html Ambion tips on RNA extraction] | |||
Revision as of 11:07, 14 February 2008
RNA extraction with TRIZOL (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extraction from cells. It takes slightly longer than column-based methods like RNAeasy but it has higher capacity and can yield more RNA.
Principle
Reagents
Steps
Common mistakes
See also
- RNA extraction (central, general page)
External links
Reagents
- TRIZOL reagent (Invitrogen), TRIZOL on wikipedia
- TRI reagent (Sigmal Aldrich)
Protocols
- RNA extraction with TRIZOL (Stanford)
- RNA extraction with TRIZOL (Uni Florida)
- Troubleshooting guide for TRIZOL extraction (Uni Toronto)
Tips
