Questions and Answers: Difference between revisions
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==RBS prediction== | ==RBS prediction== | ||
Is there an online or otherwise easy to use tool for finding RBS sites? | Is there an online or otherwise easy to use tool for finding RBS sites? | ||
===Reply=== | |||
I've used [http://www.tigr.org/software/genefinding.shtml TIGR's software] to do RBS prediction. The program is called RBS finder. Also, if you have a specific sequence you want to test, you can use Stormo's work: | |||
<biblio> | |||
#stormo1994 pmid=8165145 | |||
</biblio> | |||
Of couse, this is all for ''E. coli'' | |||
--[[User:Skosuri|Sri Kosuri]] 19:22, 23 March 2006 (EST) | |||
==Homologous recombination in ''E. coli''== | ==Homologous recombination in ''E. coli''== | ||
Revision as of 17:22, 23 March 2006
RBS prediction
Is there an online or otherwise easy to use tool for finding RBS sites?
Reply
I've used TIGR's software to do RBS prediction. The program is called RBS finder. Also, if you have a specific sequence you want to test, you can use Stormo's work:
- Barrick D, Villanueba K, Childs J, Kalil R, Schneider TD, Lawrence CE, Gold L, and Stormo GD. Quantitative analysis of ribosome binding sites in E.coli. Nucleic Acids Res. 1994 Apr 11;22(7):1287-95. DOI:10.1093/nar/22.7.1287 |
Of couse, this is all for E. coli --Sri Kosuri 19:22, 23 March 2006 (EST)
Homologous recombination in E. coli
If anyone has experience with homologous recombination in E. coli please check out the Standard E. coli Strain for BioBricks project and annotate the page with your feedback and comments. Please include any protocols or materials information you might have on the appropriate pages and link to them from the standard strain project page as well. Any and all advice is appreciated.
Reply
See Standard E. coli Strain for BioBricks for comments. Can add more detail later.--Kathleen
