Difference between revisions of "QIAquick PCR purification"

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[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]
 
[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]
  
==Tips==
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==Notes==
You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge).  This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter.  Courtesy of [[User:Meaganl|Meagan Lizarazo]].
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*You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge).  This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter.  Courtesy of [[User:Meaganl|Meagan Lizarazo]].

Revision as of 10:15, 9 February 2007

Kit manual

Notes

  • You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazo.