QIAquick PCR purification: Difference between revisions
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Barry Canton (talk | contribs) (New page: [http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual] ==Tips== You can obtain a higher yield by doing the first spi...) |
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==Tips== | ==Tips== | ||
You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl]]. | You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl|Meagan Lizarazzo]]. | ||
Revision as of 10:15, 9 February 2007
Tips
You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazzo.
