Difference between revisions of "Protein blot (Western) hub"

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[[Image:Hub icon.png|right]]
 
[[Image:Hub icon.png|right]]
  
The '''Western blot''', also known as the immunoblot or the protein blot, is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
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The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
  
==General Western blot protocol==
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==General protein blot protocol==
* [[Western Blot (immunoblot)]]
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* [[Protein blot (Western)]]
  
 
==Western blot protocols by individual labs==
 
==Western blot protocols by individual labs==
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==See also==
 
==See also==
 
* [[Griffin:Membrane Stripping and Reprobing]]
 
* [[Griffin:Membrane Stripping and Reprobing]]
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* [[Griffin:Western Blot Optimization]]
  
 
==External links==
 
==External links==
 
* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
 
* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
 
* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
 
* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
* [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]
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* [[Image:3stars.png]] [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]
  
 
[[Category:Protocol]]  
 
[[Category:Protocol]]  
 
[[Category:In vitro]]  
 
[[Category:In vitro]]  
 
[[Category:Protein]]
 
[[Category:Protein]]

Latest revision as of 01:17, 27 June 2012

Hub icon.png

The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

General protein blot protocol

Western blot protocols by individual labs

See also

External links