Difference between revisions of "Prince:Proteomics"

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(Experiment Design)
(Experiment Design)
 
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[[Prince:Strong Cation Exchange| SCX (Strong Cation Exchange)]]
 
[[Prince:Strong Cation Exchange| SCX (Strong Cation Exchange)]]
  
[[Prince:Phospho-protein Enrichment|Phospho-protein Enrichment]]
+
[[Prince:Phosphopeptide Enrichment|Phosphopeptide Enrichment]]
  
 
[[Prince:Mass Spec|Mass Spectrometry]]
 
[[Prince:Mass Spec|Mass Spectrometry]]

Latest revision as of 12:51, 4 February 2012

Introduction

Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.

Experiment Design

File:Work flow.jpeg

Cell Lysis

FASP (Filter-aided Sample Preparation)

SCX (Strong Cation Exchange)

Phosphopeptide Enrichment

Mass Spectrometry

Protein Quantitation

Results

Yeast Proteome Coverage

UGP1 Coverage

PASK Coverage