Difference between revisions of "Prince:Proteomics"

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(Introduction)
(Experiment Design)
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[[Prince:Mass Spec|Mass Spectrometry]]
 
[[Prince:Mass Spec|Mass Spectrometry]]
  
[[Prince:Protein Concentration|Protein Concentration]]
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[[Prince:Protein Quantitation|Protein Quantitation]]
  
 
=Results=
 
=Results=

Revision as of 12:50, 4 February 2012

Introduction

Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.

Experiment Design

File:Work flow.jpeg

Cell Lysis

FASP (Filter-aided Sample Preparation)

SCX (Strong Cation Exchange)

Phospho-protein Enrichment

Mass Spectrometry

Protein Quantitation

Results

Yeast Proteome Coverage

UGP1 Coverage

PASK Coverage