Prince:Mass Spectrometry
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New Users
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Introduction to Mass Spectrometry
Sample Preparation
Proven Methods:
- FASP (filter aided sample preparation)
- In Gel FASP
- Yeast FASP
Side Notes:
Wet Lab Rules
Equipment
Gases
[From the wall out]
- Helium UHP [LTQ] (40psi)
- Nitrogen UHP [HCD] (100psi)
- Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
- Nitrogen Normal (100psi) [for UPLC]
UPLC
- Troubleshooting the Eksigent UPLC system
- UPLC (Fittings/tubing/flow rates)
- 10 micron needles provide best nano-spray at 200-300 nl/min
Tuning/Calibration
Mascot Restart
user@jp1:~$ ls /etc/init.d/mascot /etc/init.d/mascot user@jp1:~$ sudo /etc/init.d/mascot status [sudo] password for user: user@jp1:~$ sudo /etc/init.d/mascot restart * Mascot monitor is not running * Mascot monitor started user@jp1:~$
Scheduling
You may access the scheduling site here: