Difference between revisions of "Prince:BYU Lipidomics Team"

From OpenWetWare
Jump to navigationJump to search
(Special Journal Issues on Lipidomics)
Line 75: Line 75:
''Journal of Chromatography B'' [http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%237220%232009%23991229973%231383050%23FLA%23&_cdi=7220&_pubType=J&_auth=y&_acct=C000021830&_version=1&_urlVersion=0&_userid=456938&md5=fceed4eb1611cde1e48a2b25f4ff62d4]
''Journal of Chromatography B'' [http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%237220%232009%23991229973%231383050%23FLA%23&_cdi=7220&_pubType=J&_auth=y&_acct=C000021830&_version=1&_urlVersion=0&_userid=456938&md5=fceed4eb1611cde1e48a2b25f4ff62d4]
''Prostaglandins and Other Lipid Mediators'' [http://www.sciencedirect.com/science?_ob=PublicationURL&_cdi=4947&_pubType=J&_acct=C000021830&_version=1&_urlVersion=0&_userid=456938&md5=8cad50d7658cd70048a44f088a20f87e&jchunk=77#77]
''Prostaglandins and Other Lipid Mediators'', Volume 77, 2005 [http://www.sciencedirect.com/science?_ob=PublicationURL&_cdi=4947&_pubType=J&_acct=C000021830&_version=1&_urlVersion=0&_userid=456938&md5=8cad50d7658cd70048a44f088a20f87e&jchunk=77#77]
''European Journal of Lipid Science and Technology'' [http://onlinelibrary.wiley.com/doi/10.1002/ejlt.v111:1/issuetoc]
''European Journal of Lipid Science and Technology'', Volume 111, Issue 1, January 2009 [http://onlinelibrary.wiley.com/doi/10.1002/ejlt.v111:1/issuetoc]
==Group Members==
==Group Members==

Revision as of 12:51, 20 June 2011

Prince lab logo.png

Home        Lab Members         Research         Publications         Internal         Mass Spec         Contact        

Basic Protocol

  • Get sample from almost any source
  • Extraction (both methods use Folch reagent [2:1 chloroform:methanol])
    • use Kaluzne method
    • trying to implement Bligh & Dyer (gold standard)
    • MTBE Extraction [1]
  • Mass Spectrometry
    • ESI-Agilent TOF
    • Tandem MS-Q-Star (8-10am)
  • Analysis
    • Matlab (particular toolbox?) PCA analysis

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.


Lipid Signalling

Sphingolipid signalling. [2]

Lipid mediators in health and disease. [3]

Lipid mediators for inflammation resolution. [4]

Mass Spectrometry

Practical essentials of ESI.* [5]

Solid tutorial review on mass spec "-omics."* [6]

Recent advances (2011) in MS lipidomics. [7]

General aspects of lipidomics. [8]

Basics of ESI and intrasource separation shotgun lipidomics.** [9]

Tissue sample preparation for intrasource separation.** [10]

AMDMS-SL. [11]

Top-down lipidomic strategy.** [12]

Blood plasma and top-down lipidomics.* [13]

Yeast quantitative shotgun lipidomics. [14] [15]

Eicosanoid lipidomics. [16]

Bioactive lipidomics. [17]

Lipid mediator informatics-lipidomics. [18]

Mediator lipidomics. [19]


Chemometrics. [20] [21]

S-plot. [22]

PCA package. [23]

K-OPLS package. [24]

Molecular fragmentation query language. [25]

Special Journal Issues on Lipidomics

Journal of Chromatography B [26]

Prostaglandins and Other Lipid Mediators, Volume 77, 2005 [27]

European Journal of Lipid Science and Technology, Volume 111, Issue 1, January 2009 [28]

Group Members


  • Mary Blackburn
  • Mackay Merrill
  • Dean Flanders
  • Landon Weist
  • Rob Roden
  • Greg Jackson
  • Daniel Edmondson
  • Matthew Linford
  • Craig Thulin
  • Steven Wood
  • John Prince

Former or Associated

  • Jennifer MacDonald
  • Jonathan Lee
  • Vinod Chaudhary
  • Steven Herron

Relevant Publications

  1. Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, Fantin VR, Jang HG, Jin S, Keenan MC, Marks KM, Prins RM, Ward PS, Yen KE, Liau LM, Rabinowitz JD, Cantley LC, Thompson CB, Vander Heiden MG, and Su SM. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature. 2009 Dec 10;462(7274):739-44. DOI:10.1038/nature08617 | PubMed ID:19935646 | HubMed [P1]

    This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.