Preparing chemically competent cells
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- Grow a 5-25mL culture to an OD600 of 0.2 [math]\displaystyle{ \rightarrow }[/math] 0.5
- Centrifuge for 10 minutes at 3000rpm and [math]\displaystyle{ 4^o }[/math]C.
- Remove supernatant and replace with 10% original volume TSS.
- Make 100uL aliquots and store at [math]\displaystyle{ -80^o }[/math]C.