Preparing chemically competent cells

From OpenWetWare
Jump to: navigation, search
  • Grow a 5-25mL culture to an OD600 of 0.2 Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \rightarrow} 0.5
  • Centrifuge for 10 minutes at 3000rpm and Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle 4^o} C.
  • Remove supernatant and replace with 10% original volume TSS.
  • Make 100uL aliquots and store at Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle -80^o} C.