Prbbbb:Coomassi Ethanol stain

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Revision as of 03:19, 6 August 2009 by Raik (talk | contribs) (New page: Ethanol based variant of the Coomassi protein stain solution. ==Mix== * 40 % Ethanol * 7 % Acetic Acid * 0.1 % w/v Coomassi Brilliant Blue-R * double-distilled water ==Usage== # Stain...)
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Ethanol based variant of the Coomassi protein stain solution.

Mix

  • 40 % Ethanol
  • 7 % Acetic Acid
  • 0.1 % w/v Coomassi Brilliant Blue-R
  • double-distilled water

Usage

  1. Stain at room temperature for 1h or until overnight
  2. De-stain 30 min with Prbbbb:Coomassi Ethanol destain

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

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