Difference between revisions of "PrbbBB:Protocols"

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(Protein Expression)
 
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* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
 
* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
 
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
 
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
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* [[PrbbBB:Oligo_Annealing|Annealing of complementary long Oligos]]
  
 
==Protein Expression==
 
==Protein Expression==
  
 
* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]]
 
* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]]
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* [[Prbbbb:large_scale_expression_v1|Large-scale expression in BL21]]
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* [[Prbbbb:inclusion_body_solubilization_v1|Solubilization of E. coli inclusion bodies]]
 
* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]]
 
* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]]
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 +
==Protein measurements==
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* [[Prbbbb:in_vitro_FRET_FRB_FKBP_v1|''in-vitro'' FRET measurements]]
  
 
[[Category:Protocol]]
 
[[Category:Protocol]]

Latest revision as of 06:11, 8 August 2010

PrbbBB.jpg

<html> <div style="padding: 2px; color: #000; background-color: #969594; width: 766px"> <table> <colgroup width=126 span=6> </colgroup>

   	<tr>
       	<td width=120><a href="PrbbBB">Home</td>
       	<td width=120><a href="http://openwetware.org/wiki/PrbbBB:Members">Members</td>
       	<td width=120><a href="http://openwetware.org/wiki/PrbbBB:Protocols">Protocols</td>

<td width=120><a href="http://openwetware.org/wiki/PrbbBB:Internal">Internal</td> <td width=120><a href="http://openwetware.org/wiki/PrbbBB:Contact">Contact</td>

     	</tr>

</table> </div> </html>

Biobrick assembly & related

We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.

Protein Expression

Protein measurements