Difference between revisions of "Prather:Protocols"

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This page contains protocols and documents used by the Prather Lab at MIT.
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===Prather Lab Protocols===
 
 
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 6-25-07)
 
*[[media:Advanced_Protocols.doc|Advanced Molecular Biology Protocols]]  (As of 12-16-06)
 
*[[media:Guide_to_the_Prather_Lab.doc|Prather Lab New Student Guide]]  (As of 12-16-06)
 
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]]
 
*[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]]
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
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*[[media:New_Member_Guide_-_6June11.pdf‎|Prather Lab New Student Guide]]  (PDF, Updated June 2011)
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*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (PDF, Updated 15Mar2007)
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*[[media:Prather_Lab_Experimental_Protocols_25Jan09.pdf|Basic Molecular Biology Protocols]] (PDF, Updated Jan. 2009)
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**Growing ''E. coli'' in liquid culture and on solid media
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**DNA electrophoresis
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**Gel extraction
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**PCR
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**DNA digestion with restriction enzymes
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**DNA ligation reaction
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**Electrocompetent transformations
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**Preparation of freezer stocks
  
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*[[media:Advanced_Protocols_2009.pdf|Advanced Molecular Biology Protocols]]  (PDF, Updated Jan. 2009)
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**Preparation of bacterial lysates by freeze-thaw
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**Bradford total protein assay
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**Boiling-lysis preparation of pDNA
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**Sample dialysis
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**Running a protein gel
  
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*[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]]
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*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]]  (PDF, Updated Feb. 2009)
  
 
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[[Prather | Back to Prather Lab Home]]
 
[[Prather | Back to Prather Lab Home]]

Revision as of 09:26, 6 June 2011

Prather Lab Protocols

  • Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
    • Preparation of bacterial lysates by freeze-thaw
    • Bradford total protein assay
    • Boiling-lysis preparation of pDNA
    • Sample dialysis
    • Running a protein gel

Back to Prather Lab Home