Prather:Protocols: Difference between revisions
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===Prather Lab Protocols and Useful Documents=== | ===Prather Lab Protocols and Useful Documents=== | ||
*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]] (As of 14Aug2007) | |||
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] | |||
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007) | *[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007) | ||
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**Running a protein gel | **Running a protein gel | ||
*[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]] | *[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]] | ||
*[[media:Undergraduate_Student_Evaluation_Form.doc|Undergraduate Student Evaluation Form]] | *[[media:Undergraduate_Student_Evaluation_Form.doc|Undergraduate Student Evaluation Form]] | ||
Revision as of 10:31, 24 August 2007
Prather Lab Protocols and Useful Documents
- Prather Lab New Student Guide (As of 14Aug2007)
- Good Laboratory Practice Handout
- Basic Molecular Biology Protocols (As of 25Jun2007)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent and chemical transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (As of 16Dec2006)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
