Difference between revisions of "Prather:HPLC"

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(Maintenance Log)
(Maintenance Log)
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==Maintenance Log==
==Maintenance Log==
A maintenance logbook is in the drawer under the HPLC.  Please record the date and a description of any maintenance you perfom.
A maintenance logbook is on the shelf above the HPLC.  Please record the date and a description of any maintenance you perfom.
==Reserving Time on the HPLC==
==Reserving Time on the HPLC==

Revision as of 08:38, 25 February 2010

Important Update

Transition to Single Column System

As of August 14, 2007 the HPLC is set up as a single column system to accomodate backflushing procedures for the Aminex column. This means that only one column can be installed at a time. The column connectors and flow direction are marked on the HPLC tubing.

With this new system configuration, ALL users will need to modify their methods as follows:

  • Reconfigure the column/thermostat to 'Backflushing off' rather than simply 'Column 1' or 'Column 2'
  • Set the thermostat for both sides of the heating block if your column straddles both of them.
  • The new plumbing may cause the retention times of your peaks to shift, so you may want to consider constructing a new calibration curve

Aminex Column Updates

  • The method AMINEXBACKFLUSH.M can be used to automatically backflush the column
    • When running supernatants or samples containing salts using this method, run a blank every 10-15 samples
    • The method's run time is 45 min
    • This method should allow users to run > 15 samples without loss of resolution or the need to perform a manual backflush
  • A guard column has been purchased specifically for the Aminex column
    • Please use the guard to protect the analytical column and extend its life
    • Install and remove the guard column and analytical column as a single unit

Column Installation & Storage Notes

Now that the HPLC is a single column system, it is important to be aware of what mobile phase is in the system before starting your analysis. Here are some guidelines that will help protect the integrity of the columns and your data.

  • Before installing your column, flush the entire HPLC system with water with no column installed to remove the previous user's mobile phase
  • When removing another user's column always cap the ends to prevent drying and store it in its original box
  • If your column requires special storage conditions it is your responsibility to flush it with the appropriate storage buffer and remove it from the HPLC at the end of your reservation

Maintenance Log

A maintenance logbook is on the shelf above the HPLC. Please record the date and a description of any maintenance you perfom.

Reserving Time on the HPLC

The HPLC in the Prather Lab is currently in high demand. We've set up an electronic calendar to ensure that everyone has enough time to use the instrument. The calendar is managed through Google calendar, and you can access the calendar to reserve the HPLC ahead of time by following the steps below:

  • If you have a Google account, subscribe to the Prather Lab HPLC calendar by clicking on the button below.

<html><a target="_blank" href="http://www.google.com/calendar/render?cid=bcbp49couugtcksadk4j7kcat4%40group.calendar.google.com"><img src="http://www.google.com/calendar/images/ext/gc_button1_en.gif" border=0></a></html>

  • If you don't have a Google account, you can sign up for one using an existing email address by clicking here.
  • Once you have a Google account and have subscribed to the calendar, email dmbower@mit.edu with your Google username if you would like edit access. With edit access, you can use the calendar to reserve the HPLC.

HPLC Schedule

<html><iframe src="http://www.google.com/calendar/embed?src=bcbp49couugtcksadk4j7kcat4%40group.calendar.google.com&amp;chrome=NAVIGATION&amp;height=588" style=" border-width:0 " width="640" frameborder="0" height="588"></iframe></html>

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