From OpenWetWare
Revision as of 07:30, 18 July 2005 by Ssutton (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

What is it?

• a Fusion of a Pyrococcus-like enzyme (Pfu) with a double-strand DNA binding domain → increased processivity.

Stats about Phusion™ High-Fidelity DNA Polymerase:

• 10x processivity compared to Taq • 50x fidelity compared to Taq • creates blunt end • error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer • non-displacing