Difference between revisions of "Phusion"

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'''What is it?'''
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==Description==
  
• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
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Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
  
'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
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==Statistics about Phusion™ High-Fidelity DNA Polymerase==
 
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*10x processivity compared to Taq
10x processivity compared to Taq
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*50x fidelity compared to Taq
 
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*Creates blunt end
50x fidelity compared to Taq
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*Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 
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*Non-displacing
• creates blunt end
 
 
 
• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 
 
 
• non-displacing
 
 
 
'''Links'''
 
  
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==References==
 
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
 
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
 
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]
 
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]

Revision as of 11:23, 13 September 2006

Description

Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Statistics about Phusion™ High-Fidelity DNA Polymerase

  • 10x processivity compared to Taq
  • 50x fidelity compared to Taq
  • Creates blunt end
  • Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
  • Non-displacing

References