Phusion: Difference between revisions

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'''What is it?'''
==Description==


• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.


'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
==Statistics about Phusion™ High-Fidelity DNA Polymerase==
 
*10x processivity compared to Taq
• 10x processivity compared to Taq
*50x fidelity compared to Taq
 
*Creates blunt end
• 50x fidelity compared to Taq
*Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 
*Non-displacing
• creates blunt end
 
• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 
• non-displacing
 
'''Links'''


==References==
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]

Revision as of 12:23, 13 September 2006

Description

Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Statistics about Phusion™ High-Fidelity DNA Polymerase

  • 10x processivity compared to Taq
  • 50x fidelity compared to Taq
  • Creates blunt end
  • Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
  • Non-displacing

References

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