Difference between revisions of "Phusion"

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'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
 
'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
 
• 10x processivity compared to Taq
 
• 10x processivity compared to Taq
 +
 
• 50x fidelity compared to Taq
 
• 50x fidelity compared to Taq
 +
 
• creates blunt end
 
• creates blunt end
 +
 
• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 
• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 +
 
• non-displacing
 
• non-displacing
  
Website:
+
'''Website'''
 
http://www.neb.com/nebecomm/products/productF-530.asp
 
http://www.neb.com/nebecomm/products/productF-530.asp

Revision as of 07:32, 18 July 2005

What is it?

• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Stats about Phusion™ High-Fidelity DNA Polymerase • 10x processivity compared to Taq

• 50x fidelity compared to Taq

• creates blunt end

• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer

• non-displacing

Website http://www.neb.com/nebecomm/products/productF-530.asp