Pfu

Purpose

• Pfu is a thermostable DNA polymerase that can be used for PCR.

Procurement

• You can buy Pfu for example at Promega.
• NEB does for some reason not offer Pfu.

Use

• Pfu has proof reading activity which makes it more accurate than for example Taq.
• Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.