Pfu
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Purpose
- Pfu is a thermostable DNA polymerase that can be used for PCR.
Procurement
- You can buy Pfu for example at [Promega].
- NEB does for some reason not offer Pfu.
Use
- Pfu has proof reading activity which makes it more accurate than for example Taq.
- Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.
