Pfu

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Revision as of 00:25, 14 April 2008 by Torsten Waldminghaus (talk | contribs) (New page: ==Purpose== *Pfu is a thermostable DNA polymerase that can be used for PCR. ==Procurement== *You can buy Pfu for example at [[http://www.promega.com/catalog/catalogproducts.aspx?categoryn...)
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Purpose

  • Pfu is a thermostable DNA polymerase that can be used for PCR.

Procurement

  • You can buy Pfu for example at [Promega].
  • NEB does for some reason not offer Pfu.

Use

  • Pfu has proof reading activity which makes it more accurate than for example Taq.
  • Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.