Difference between revisions of "Pfu"

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(Procurement)
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==Procurement==
 
==Procurement==
*You can buy Pfu for example at [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1311&ckt=1 Promega].
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*You can buy Pfu for example at [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1311&ckt=1 Promega] or [http://www.fermentas.com/catalog/modifyingenzymes/pfudnapolymer.htm Fermentas].
 
*NEB does for some reason not offer Pfu.
 
*NEB does for some reason not offer Pfu.
  

Revision as of 10:56, 23 April 2008

Purpose

  • Pfu is a thermostable DNA polymerase that can be used for PCR.

Procurement

  • You can buy Pfu for example at Promega or Fermentas.
  • NEB does for some reason not offer Pfu.

Use

  • Pfu has proof reading activity which makes it more accurate than for example Taq.
  • Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.