Difference between revisions of "Pecinka lab:Protocols"

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[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>]
 
[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>]
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RNA work
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[[media:RNA_gels.pdf| RNA gels]]
  
 
[[Pecinka_Lab:Trizol|<font style="color:#000;">Trizol extraction</font>]]
 
[[Pecinka_Lab:Trizol|<font style="color:#000;">Trizol extraction</font>]]

Revision as of 08:20, 18 February 2013

Pecinka group2.jpg


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Protocols

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

RNA work

RNA gels

Trizol extraction

Stocks

Antibiotics

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl2

Lab Safety

Pecinka lab safety

Lab Tasks

Scripts, instructions and miscellaneous

Analyzing luminiscence over time with ImageJ

HALLE 14 growth chambers