Difference between revisions of "Pecinka lab:Protocols"

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RNA gels [[media:RNA_gels.pdf| (PDF)]]
 
RNA gels [[media:RNA_gels.pdf| (PDF)]]
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'''Histochemical staining'''
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GUS staining [[media:GUS_staining_OWW.pdf| (PDF)]]
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Alexander staining for pollen viability [[media:Alexander_staining.pdf| (PDF)]]
  
 
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Latest revision as of 08:19, 17 July 2013

Pecinka group2.jpg


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Protocols

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

RNA work

RNeasy kit extraction (PDF)

Trizol extraction (PDF)

RNA gels (PDF)

Histochemical staining

GUS staining (PDF)

Alexander staining for pollen viability (PDF)

Stocks

Antibiotics

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl2

Lab Safety

Pecinka lab safety

Lab Tasks

Scripts, instructions and miscellaneous

Analyzing luminiscence over time with ImageJ

HALLE 14 growth chambers