Pecinka lab:Protocols: Difference between revisions
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[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>] | [http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>] | ||
RNA work | '''RNA work''' | ||
[[media: | RNeasy kit extraction [[media:RNeasy_plant.pdf| (PDF)]] | ||
[[ | Trizol extraction [[media:TRIZOL.pdf| (PDF)]] | ||
RNA gels [[media:RNA_gels.pdf| (PDF)]] | |||
'''Histochemical staining''' | |||
GUS staining [[media:GUS_staining_OWW.pdf| (PDF)]] | |||
Alexander staining for pollen viability [[media:Alexander_staining.pdf| (PDF)]] | |||
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Latest revision as of 08:19, 17 July 2013
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Centre of Plant Structural and Functional Genomics |
ProtocolsMiniprep using Promega reagents but not the columns Eleven Golden Rules of qRT-PCR (Uvardi et al.) RNA work RNeasy kit extraction (PDF) Trizol extraction (PDF) RNA gels (PDF) Histochemical staining GUS staining (PDF) Alexander staining for pollen viability (PDF) |
Stocks |
Lab Safety |
Lab Tasks |
Scripts, instructions and miscellaneous |
