From OpenWetWare
Revision as of 14:27, 1 September 2009 by Eric Milliman (talk | contribs) (fixed typos)
Jump to: navigation, search


PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.

Molecular W.: 174.19


You can buy PMSF for example at Pierce or Sigma.


PMSF is not soluble in water, dissolve in ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C. A common final concentration of PMSF is 1mM of course depending on the experimental setup.


PMSF is poisonous and should be handled carefully.