Difference between revisions of "PMSF"

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==Purpose==
 
==Purpose==
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.
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PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.
  
 
Molecular W.: 174.19
 
Molecular W.: 174.19
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==Use==
 
==Use==
Don't try solution in water, it does not work. Take ethanol or methanol.
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PMSF is not soluble in water, dissolve in ethanol or methanol.
200 mM stock solution are stable for months at 4°C.
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200 mM stock solution (0.035 g/mL) is stable for months at 4°C.
A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.
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A common final concentration of PMSF is 1mM of course depending on the experimental setup.
  
 
==Safety==
 
==Safety==
PMSF is poisenes and should be handeld carefully.
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PMSF is poisonous and should be handled carefully.
  
  
 
[[Category:Material]] [[Category:Chemical]]
 
[[Category:Material]] [[Category:Chemical]]

Revision as of 14:27, 1 September 2009

Purpose

PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.

Molecular W.: 174.19

Procurement

You can buy PMSF for example at Pierce or Sigma.

Use

PMSF is not soluble in water, dissolve in ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C. A common final concentration of PMSF is 1mM of course depending on the experimental setup.

Safety

PMSF is poisonous and should be handled carefully.