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Revision as of 07:57, 31 August 2006 by Crp724 (talk | contribs) (Making 2x PLS)
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Making 2x PLS

1M Tris pH 6.8 1.5ml

10% SDS 6.0ml

Glycerol 3.0ml

H20 3.0ml

Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.