From OpenWetWare
Revision as of 07:57, 31 August 2006 by Crp724 (talk | contribs) (Making PLS)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Making 2x PLS

1M Tris pH 6.8 1.5ml 10% SDS 6.0ml Glycerol 3.0ml H20 3.0ml Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.