Difference between revisions of "PLS"

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(Making PLS)
 
(Making 2x PLS)
Line 1: Line 1:
 
 
== Making 2x PLS ==
 
== Making 2x PLS ==
  
  
 
1M Tris pH 6.8          1.5ml
 
1M Tris pH 6.8          1.5ml
 +
 
10% SDS                6.0ml
 
10% SDS                6.0ml
 +
 
Glycerol                3.0ml
 
Glycerol                3.0ml
 +
 
H20                    3.0ml
 
H20                    3.0ml
 +
 
Bromophenol blue        small dash to color
 
Bromophenol blue        small dash to color
  

Revision as of 07:57, 31 August 2006

Making 2x PLS

1M Tris pH 6.8 1.5ml

10% SDS 6.0ml

Glycerol 3.0ml

H20 3.0ml

Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.