Difference between revisions of "PLS"

From OpenWetWare
Jump to: navigation, search
(Making 2x PLS)
(Making 2x PLS)
Line 2: Line 2:
  
  
1M Tris pH 6.8          1.5ml
+
*1M Tris pH 6.8          1.5ml
 
+
*10% SDS                6.0ml
10% SDS                6.0ml
+
*Glycerol                3.0ml
 
+
*H20                    3.0ml
Glycerol                3.0ml
+
*Bromophenol blue        small dash to color
 
 
H20                    3.0ml
 
 
 
Bromophenol blue        small dash to color
 
  
 
vortex, aliquot and freeze at -20
 
vortex, aliquot and freeze at -20

Revision as of 07:58, 31 August 2006

Making 2x PLS

  • 1M Tris pH 6.8 1.5ml
  • 10% SDS 6.0ml
  • Glycerol 3.0ml
  • H20 3.0ml
  • Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.