Difference between revisions of "PIPES"

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piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757
 
piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757
  
A Good buffer <cite>Good66</cite><cite>Blanchard84</cite>.
+
A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>.
  
 
* pK<sub>a</sub> at 25C of 6.8 (a second one at pH 3 is not of interest)
 
* pK<sub>a</sub> at 25C of 6.8 (a second one at pH 3 is not of interest)
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<biblio>
 
<biblio>
 
# Good66 pmid=5942950
 
# Good66 pmid=5942950
 +
# Good74 pmid=4206745
 
# Blanchard84 pmid=6717292  
 
# Blanchard84 pmid=6717292  
 
</biblio>
 
</biblio>
 +
 +
[[Category:Material]] and [[Category:Buffers]]

Latest revision as of 13:33, 11 March 2008

piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757

A Good buffer [1][2][3].

  • pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
  • usable buffering range of 6.1 to 7.5
  • molecular weight 302.4
  • ΔpKa/ΔT = -.0085

Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. PubMed ID:5942950 | HubMed [Good66]
  2. Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53-68. PubMed ID:4206745 | HubMed [Good74]
  3. Blanchard JS. Buffers for enzymes. Methods Enzymol. 1984;104:404-14. PubMed ID:6717292 | HubMed [Blanchard84]
All Medline abstracts: PubMed | HubMed and