Difference between revisions of "PCR"

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(Specific Protocols)
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==Specific Protocols==
 
==Specific Protocols==
[[Silver: PCR]]
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*[[Silver: PCR]]
 
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*[[E. huxleyi PCR]]
[[E. huxleyi PCR]]
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*[[Knight:Colony PCR]]
  
 
==Notes==
 
==Notes==
#Here is a paper ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14511688&query_hl=1 pubmed], [http://www.gene-quantification.de/arezi-2003.pdf pdf], [http://dx.doi.org/10.1016/S0003-2697(03)00465-2 doi]) which discusses the amplification efficiencies of different DNA polymerases on templates of varying length and GC content using real-time PCR.
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#A discussion of the amplification efficiencies of different DNA polymerases on templates of varying length and GC content using real-time PCR <cite>Arezi-AnalBiochem-2003</cite>.
  
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==References==
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<biblio>
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#Arezi-AnalBiochem-2003 pmid=14511688
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</biblio>
  
 
[[Category:Protocol]]
 
[[Category:Protocol]]
 
[[Category:DNA]]
 
[[Category:DNA]]
 
[[Category:In vitro]]
 
[[Category:In vitro]]

Revision as of 14:36, 14 September 2006

General Information

PCR is an acronym for polymerase chain reaction. It is a method for amplifying DNA in vitro.

General Procedure

Specific Protocols

Notes

  1. A discussion of the amplification efficiencies of different DNA polymerases on templates of varying length and GC content using real-time PCR [1].

References

  1. Arezi B, Xing W, Sorge JA, and Hogrefe HH. Amplification efficiency of thermostable DNA polymerases. Anal Biochem. 2003 Oct 15;321(2):226-35. PubMed ID:14511688 | HubMed [Arezi-AnalBiochem-2003]