Difference between revisions of "PBST"

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(Purpose)
 
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== Purpose ==
 
== Purpose ==
* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
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* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
 
 
 
* PBST is used in [[protein blots]] for washing of membrane labelled with antibody.
 
* PBST is used in [[protein blots]] for washing of membrane labelled with antibody.
  

Latest revision as of 17:42, 23 December 2012

PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).

Purpose

  • PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
  • PBST is used in protein blots for washing of membrane labelled with antibody.

Recipe

0.1% (v/v) Tween 20 in 1x PBS

examples

  • 100µl in 100ml 1x PBS
  • 1ml in 1L 1xPBS