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1. Combine the following:

80 g NaCl 2 g KCl 14.4 g Na2HPO4 2.4 g KH2PO4 800 mL distilled H2O

2. Adjust pH to 7.4 with HCl

3. Add H2O to 1L

4. Autoclave for 20 minutes on liquid cycle. Store at RT.