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1. Combine the following:

80 g NaCl

2 g KCl

14.4 g Na2HPO4

2.4 g KH2PO4

800 mL distilled H2O


2. Adjust pH to 7.4 with HCl


3. Add H2O to 1L


4. Autoclave for 20 minutes on liquid cycle. Store at RT.