Difference between revisions of "PBS"

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Line 2: Line 2:
  
 
80 g NaCl
 
80 g NaCl
 +
 
2 g KCl
 
2 g KCl
 +
 
14.4 g Na2HPO4
 
14.4 g Na2HPO4
 +
 
2.4 g KH2PO4
 
2.4 g KH2PO4
 +
 
800 mL distilled H2O
 
800 mL distilled H2O
  

Revision as of 14:44, 14 June 2005

1. Combine the following:

80 g NaCl

2 g KCl

14.4 g Na2HPO4

2.4 g KH2PO4

800 mL distilled H2O

2. Adjust pH to 7.4 with HCl

3. Add H2O to 1L

4. Autoclave for 20 minutes on liquid cycle. Store at RT.