Order a Microfluidic Chip from the Foundry: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(4 intermediate revisions by 2 users not shown)
Line 5: Line 5:
#Use the Union command on the entire layer.
#Use the Union command on the entire layer.
#Use the Explode command on the entire layer.  You will likely have to do this several times, until autocad tells you that the number of objects selected is the same as the number of objects that it couldn't explode.
#Use the Explode command on the entire layer.  You will likely have to do this several times, until autocad tells you that the number of objects selected is the same as the number of objects that it couldn't explode.
#Look carefully through the entire layer.  Remove extraneous lines such that the channels are all properly enclosed areas.
#Use the Pedit command.
#Use the Pedit command.
#*Choose Multiple.
#*Choose Multiple.
Line 12: Line 13:
#*Enter a fuzz distance of 0.
#*Enter a fuzz distance of 0.
#*Hit escape so as not to repeat the process.
#*Hit escape so as not to repeat the process.
#Repeat the process for all layers.
#Repeat the process (steps 1-6) for all layers.
#Copy the layers independently, and paste them into the [[Image:Output_city_template.dwg|template file]].  The push down layers should be placed in the boxed regions (top right and bottom left), and the fluid layers in the non-boxed regions (top right and bottom left). [[Image:Chip_design2.1_-_for_outputcity.dwg|See here]] for an example.
#Copy the layers independently, and paste them into the [[Image:Output_city_template.dwg|template file]].  The push down layers should be placed in the boxed regions (top right and bottom left), and the fluid layers in the non-boxed regions (top right and bottom left). [[Image:Chip_design2.1_-_for_outputcity.dwg|See here]] for an example.
#Layers that will be made out of thick PDMS need to be expanded by 1.5%.  Select these layers (individually), and use the Scale command.  Select the center of the layer to be the reference point, and use a scale factor or 1.015.
#Layers that will be made out of thick PDMS need to be expanded by 1.5%.  Select these layers (individually), and use the Scale command.  Select the center of the layer to be the reference point, and use a scale factor or 1.015.
Line 23: Line 24:
*Need transparencies printed (8x10” with emulsion down at 20,000 dpi).
*Need transparencies printed (8x10” with emulsion down at 20,000 dpi).
*The channels should be opaque with a clear background, except for the two boxed regions (upper right and lower left) which should have the reverse polarity (opaque background and clear channels).
*The channels should be opaque with a clear background, except for the two boxed regions (upper right and lower left) which should have the reverse polarity (opaque background and clear channels).
*Bill to Purchase Order 'MIT20'.
*See [[Ty Thomson|Ty]] for the PO number to use.
*Have the masks mailed to  Alejandra Torres, California Institute of Technology, 391 S. Holliston MC 128-95, Pasadena, CA 91125
*Have the masks mailed to  Alejandra Torres, California Institute of Technology, 391 S. Holliston MC 128-95, Pasadena, CA 91125


Line 29: Line 30:
'''''Tell microfluidic foundry about the order'''''
'''''Tell microfluidic foundry about the order'''''


Talk to Alejandra (atorres@caltech.edu) about the order.  Make sure that she knows what you need.  Be nice to her, cause she makes lots of good stuff for us!
[[Category:Protocol]] [[Category:Microfluidics]]

Latest revision as of 10:55, 9 May 2007

Preparing an autocad file to be printed by CAD/Art Services.

  1. Disable all but one layer in the autocad file. Make sure that the visible layer is selected.
  2. Use the Region command on the entire layer.
  3. Use the Union command on the entire layer.
  4. Use the Explode command on the entire layer. You will likely have to do this several times, until autocad tells you that the number of objects selected is the same as the number of objects that it couldn't explode.
  5. Look carefully through the entire layer. Remove extraneous lines such that the channels are all properly enclosed areas.
  6. Use the Pedit command.
    • Choose Multiple.
    • Select the entire layer.
    • Choose 'Yes' to convert lines to polylines.
    • Choose Join.
    • Enter a fuzz distance of 0.
    • Hit escape so as not to repeat the process.
  7. Repeat the process (steps 1-6) for all layers.
  8. Copy the layers independently, and paste them into the File:Output city template.dwg. The push down layers should be placed in the boxed regions (top right and bottom left), and the fluid layers in the non-boxed regions (top right and bottom left). File:Chip design2.1 - for outputcity.dwg for an example.
  9. Layers that will be made out of thick PDMS need to be expanded by 1.5%. Select these layers (individually), and use the Scale command. Select the center of the layer to be the reference point, and use a scale factor or 1.015.
  10. Save the file as autocad version 2000 or earlier.


Submitting the file to CAD/Art Services to be printed.

Send an email to cas@outputcity.com with the following information (and autocad files attached)

  • Need transparencies printed (8x10” with emulsion down at 20,000 dpi).
  • The channels should be opaque with a clear background, except for the two boxed regions (upper right and lower left) which should have the reverse polarity (opaque background and clear channels).
  • See Ty for the PO number to use.
  • Have the masks mailed to Alejandra Torres, California Institute of Technology, 391 S. Holliston MC 128-95, Pasadena, CA 91125


Tell microfluidic foundry about the order