Difference between revisions of "ONP"

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*[[Beta-galactosidase]] cleaves [[ONPG]] to produce ONP and galactose.
 
*[[Beta-galactosidase]] cleaves [[ONPG]] to produce ONP and galactose.
 
*The molar extinction coefficient is reported to be either 4500 M<sup>-1</sup>cm<sup>-1</sup> <cite>Skoneczny-BiochemJ-2000</cite> or 4900  M<sup>-1</sup>cm<sup>-1</sup> <cite>Onishi-ApplEnvironMicrobiol-1995</cite> (not original references, better references would be preferable).
 
*The molar extinction coefficient is reported to be either 4500 M<sup>-1</sup>cm<sup>-1</sup> <cite>Skoneczny-BiochemJ-2000</cite> or 4900  M<sup>-1</sup>cm<sup>-1</sup> <cite>Onishi-ApplEnvironMicrobiol-1995</cite> (not original references, better references would be preferable).
*Slightly soluble in water (0.21 g/100ml)
+
*Slightly soluble in water (0.21 g/100ml or 2.1mg/mL)
  
 
==References==
 
==References==

Latest revision as of 08:53, 30 October 2007

o-nitrophenol

  • Beta-galactosidase cleaves ONPG to produce ONP and galactose.
  • The molar extinction coefficient is reported to be either 4500 M-1cm-1 [1] or 4900 M-1cm-1 [2] (not original references, better references would be preferable).
  • Slightly soluble in water (0.21 g/100ml or 2.1mg/mL)

References

  1. Skoneczny M and Rytka J. Oxygen and haem regulate the synthesis of peroxisomal proteins: catalase A, acyl-CoA oxidase and Pex1p in the yeast Saccharomyces cerevisiae; the regulation of these proteins by oxygen is not mediated by haem. Biochem J. 2000 Aug 15;350 Pt 1:313-9. PubMed ID:10926859 | HubMed [Skoneczny-BiochemJ-2000]
  2. Onishi N and Tanaka T. Purification and properties of a novel thermostable galacto-oligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119. Appl Environ Microbiol. 1995 Nov;61(11):4026-30. PubMed ID:8526517 | HubMed [Onishi-ApplEnvironMicrobiol-1995]
  3. Chemical information [o-nitrophenol]
All Medline abstracts: PubMed | HubMed