Nissl staining: Difference between revisions
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[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]] | [[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]] | ||
The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology. | The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology. | ||
Revision as of 05:06, 20 May 2010
The Nissl staining is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.
Principle
The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.
Target of the dye
Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid.
