Neomycin

Experimental

• target organism: bacteria, mammalian cells
• stock solution: 10mg/ml in water
• working solution: 10-50µg/ml (stable at RT for 5days)

Description

• mode of action: binds to ribosomal components and inhibits protein synthesis.
• mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.

Links

• related antibiotics: kanamycin, G418
• list of cell culture reagents by Krackeler [1]
• list of cell culture reagents by USB [2]
• forum thread on difference between neomycin & G418 [3]