Difference between revisions of "Neomycin"

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(neo - beta 3 - reformating: too many small headings)
 
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==Experimental==
 
==Experimental==
  
'''target organism''': bacteria, mammalian cells
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*'''target organism''': bacteria, mammalian cells
'''stock solution''': 10mg/ml in water
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*'''stock solution''': 10mg/ml in water
'''working solution''': 10-50µg/ml (stable at RT for 5days)
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*'''working solution''': 10-50µg/ml (stable at RT for 5days)
  
 
==Description==
 
==Description==
  
'''mode of action''': binds to ribosomal components and inhibits protein synthesis.
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*'''mode of action''': binds to ribosomal components and inhibits protein synthesis.
'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
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*'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
  
 
==Links==
 
==Links==
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* list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14]
 
* list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14]
 
* forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin]
 
* forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin]
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[[Category:Material]] [[Category:Antibiotic]]

Latest revision as of 09:47, 26 July 2007

Experimental

  • target organism: bacteria, mammalian cells
  • stock solution: 10mg/ml in water
  • working solution: 10-50µg/ml (stable at RT for 5days)

Description

  • mode of action: binds to ribosomal components and inhibits protein synthesis.
  • mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.

Links

  • related antibiotics: kanamycin, G418
  • list of cell culture reagents by Krackeler [1]
  • list of cell culture reagents by USB [2]
  • forum thread on difference between neomycin & G418 [3]