Difference between revisions of "Nelson:To Do - Sid"

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==The List==
==The List==
*make competent Xl1-blue cells
*transform dsM13 into Xl1-blue
*pour two SDS-PAGE gels (12%)
*run fractions from the polg-b mutant on SDS-PAGE
*spin down the cells in the shaker
*make 6 L of LB and autoclave, plus 4 - 100 ml flasks
*start overnight culture of RNAp (AMP/Chloro)

Revision as of 07:36, 10 April 2009

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The List