Difference between revisions of "Nelson:To Do - Sid"

From OpenWetWare
Jump to: navigation, search
m (The List)
m (The List)
Line 14: Line 14:
 
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
 
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
 
*transform RNAp into freshly made competent cells
 
*transform RNAp into freshly made competent cells
*spin down the 6L of cells found in cold room
+
 
*put away dishes
 
 
*prepare DEAE cellulose according to instructions (look for it on protocols page)
 
*prepare DEAE cellulose according to instructions (look for it on protocols page)
*make tet-LB plates <br>
 
*streak Xl1-blue onto tet-LB plate <br>
 
*prepare top-agar <br>
 

Revision as of 15:06, 7 April 2009

Home        Research        Publications        Lab Members        Positions        Contact        Links        Lab stuff       

The List

  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • prepare DEAE cellulose according to instructions (look for it on protocols page)